Peptide Synthesis and Purification
Peptide Synthesis Highlights:
- Aapptec Focus multi-peptide synthesizer
- Two synthesis scales
- HPLC Purification
- A variety of modifications can be done
- Ready-made peptides
Synthesis Scales and Pricing:
| Synthesis Scale | Amino Acid Costs | HPLC Purification Costs | Expected Yield* |
|---|---|---|---|
| 0.1 mmol | $20/amino acid | $200 | ~150 mg Crude |
| ~5-20+ mg Purified | |||
| 0.25 mmol | $25/amino acid | $200 | ~150-200 mg Crude |
| (up to 8 amino acids) | ~10-50 mg Pure | ||
| 0.25 mmol | $34/amino acid | $200 | ~200-400 mg Crude |
| ~20-60+ mg Pure | |||
| plus left over crude |
*Peptide yields will depend on length and complexity of each sequence
Do you have a quote for a smaller scale synthesis? If so, send it to me so I can see if I can alter my normal synthesis scales to accommodate you.
"Ready-made and purified" peptides:
For those of you that only need a few mgs of peptide, we are offering some “ready-made and purified” peptides. The cost per mg is much lower than what you will find outside. If there is something we don’t have in stock that you would like us to make available, let us know.
| Peptide | Peptide | Price/mg | Purity |
|---|---|---|---|
| Alpha-mating Factor Pheromone, Yeast | NH2-WHWLQLKPGQPMY-OH | $12.00 | 95%+ by RP-HPLC |
| CaM Kinase II (281-291) | NH2-MHRQETVDCLK-NH2 | $25.00 | 95%+ by RP-HPLC |
| Gp100 (25-33), human | NH2-KVPRNQDWL-OH | $20.00 | 95%+ by RP-HPLC |
| GP 33-41 LCMV | GP 33-41 LCMV | $20.00 | 95%+ by RP-HPLC |
| GP 66-77 | NH2-DIYKGVYQFKSV-OH | $20.00 | 95%+ by RP-HPLC |
| HA Peptide | NH2-YPYDVPDYA-OH | $20.00 | 95%+ by RP-HPLC |
| HER-2/neu pEC2 (420-429) | NH2-PDSLRDLSVF-OH | $10.00 | 95%+ by RP-HPLC |
| Influenza HA (110-120) | NH2-SFERFEIFPKE-OH | $15.00 | 95%+ by RP-HPLC |
| MOG 35-55 | NH2-MEVGWYRSPFSRVVHLYRNGK-OH | $25.00 | 95%+ by RP-HPLC |
| OVA (257-264) | NH2-SIINFEKL-OH | $20.00 | 95%+ by RP-HPLC |
| OVA 323-339 | NH2-ISQAVHAAHAEINEAGR-OH | $25.00 | 95%+ by RP-HPLC |
| TAT 47-57 | NH2-YGRKKRRQRRR-OH | $15.00 | 95%+ by RP-HPLC |
| Tet 1 | NH2-HLNILSTLWKYR-OH | $15.00 | 95%+ by RP-HPLC |
If you need a lot of peptide, it is cheaper to get a custom synthesis done.
Peptide Modifications:
There are many modifications available including the ones listed below. Please inquire about pricing for any other unusual modifications.
| Modification | Cost |
|---|---|
| N-terminal Acetylation | No Charge |
| N-terminal Biotinylation | $30 |
| N-terminal Myristylation | $30 |
| N-terminal FITC + Linker (Ahx) | $100 + $20 |
| Ahx Linker | $30.00 |
| Phos-Serine | $100 |
| Phos-Tyrosine | $75 |
| Phos-Threonine | $100 |
| KLH conjugation via Cys Residue only | $200 |
I also have experience making Glycine branched peptides as well as O–GlcNAc-modified peptides
Please feel free to contact us: Lab: (410) 955-2739 | e-mail: syn_seq@jhmi.edu