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Peptide Synthesis and Purification

Peptide Synthesis Highlights:

  • Aapptec Focus multi-peptide synthesizer
  • Two synthesis scales
  • HPLC Purification
  • A variety of modifications can be done
  • Ready-made peptides
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Synthesis Scales and Pricing:

Synthesis Scale Amino Acid Costs HPLC Purification Costs Expected Yield*
0.1 mmol$20/amino acid$200 ~150 mg Crude
~5-20+ mg Purified
0.25 mmol$25/amino acid$200 ~150-200 mg Crude
(up to 8 amino acids)~10-50 mg Pure
0.25 mmol$34/amino acid$200 ~200-400 mg Crude
~20-60+ mg Pure
plus left over crude

*Peptide yields will depend on length and complexity of each sequence

Do you have a quote for a smaller scale synthesis?  If so, send it to me so I can see if I can alter my normal synthesis scales to accommodate you.  

"Ready-made and purified" peptides:

For those of you that only need a few mgs of peptide, we are offering some “ready-made and purified” peptides.  The cost per mg is much lower than what you will find outside.  If there is something we don’t have in stock that you would like us to make available, let us know.

PeptidePeptide Price/mg Purity
Alpha-mating Factor Pheromone, YeastNH2-WHWLQLKPGQPMY-OH $12.00 95%+ by RP-HPLC
CaM Kinase II (281-291)NH2-MHRQETVDCLK-NH2 $25.00 95%+ by RP-HPLC
Gp100 (25-33), humanNH2-KVPRNQDWL-OH $20.00 95%+ by RP-HPLC
GP 33-41 LCMVNH2-KAVYNFATM-OH $20.00 95%+ by RP-HPLC
GP 66-77NH2-DIYKGVYQFKSV-OH$20.00 95%+ by RP-HPLC
HA PeptideNH2-YPYDVPDYA-OH$20.00 95%+ by RP-HPLC
HER-2/neu pEC2 (420-429)NH2-PDSLRDLSVF-OH$15.00 95%+ by RP-HPLC
Influenza HA (110-120)NH2-SFERFEIFPKE-OH$15.00 95%+ by RP-HPLC
MOG 35-55NH2-MEVGWYRSPFSRVVHLYRNGK-OH$25.00 95%+ by RP-HPLC
OVA (257-264)NH2-SIINFEKL-OH$20.00 95%+ by RP-HPLC
OVA 257-264 A2 VarientNH2-SAINFEKL-OH$20.0095%+ by RP-HPLC
OVA 257-264 Y3 VarientNH2-SIYNFEKL-OH$20.0095%+ by RP-HPLC
OVA 257-264 G4 VarientNH2-SIIGFEKL-OH$20.0095%+ by RP-HPLC
OVA 257-264 V4 VarientNH2-SIIVFEKL-OH$20.00
(limited amount)
95%+ by RP-HPLC
OVA 257-264 Q4 VarientNH2-SIIQFEKL-OH$20.00 (limited amount)95%+ by RP-HPLC
OVA 323-339NH2-ISQAVHAAHAEINEAGR-OH$25.00 95%+ by RP-HPLC
TAT 47-57NH2-YGRKKRRQRRR-OH$15.00 95%+ by RP-HPLC
Tet 1NH2-HLNILSTLWKYR-OH$15.0095%+ by RP-HPLC
SARS-CoV-2 Spike (996-1004)NH2-LITGRLQSL-OH$15.0095%+ by RP-HPLC
SARS-CoV-2-Spike (269-277)NH2-YLQPRTFLL-OH$15.0095%+ by RP-HPLC
SARS-CoV-2-Spike (1000-1008)NH2-RLQSLQTYV-OH$15.0095%+ by RP-HPLC
HPV16 E7NH2-YMLDLQPET-OH$15.0095%+ by RP-HPLC
V5 Peptide (SV5 95-108)NH2-GKPIPNPLLGLDST-OH$20.0095%+ by RP-HPLC

If you need a lot of peptide, it is cheaper to get a custom synthesis done.

Peptide Modifications:

There are many modifications available including the ones listed below. Please inquire about pricing for any other unusual modifications.

ModificationCost
N-terminal AcetylationNo Charge
N-terminal Biotinylation$30
N-terminal Myristylation$30
N-terminal FITC + Linker (Ahx)$100 + $20
Ahx Linker$30.00
Phos-Serine$100
Phos-Tyrosine$75
Phos-Threonine$100
KLH conjugation via
Cys Residue only
$200

I also have experience making Lysine branched peptides as well as OGlcNAc-modified peptides

 

 

Please feel free to contact us: Lab: (410) 955-2739 | e-mail: syn_seq@jhmi.edu