DNA Synthesis and Purification
Oligonucleotide Synthesis Highlights:
- Oligos are synthesized and purified in our lab
- Turnaround time is usually the next day for crude oligos – depending on when the sequence is received
- HPLC purification is available
- We can help you design your primers at no charge but the oligos must be made in our lab.
- Oligo orders delivered with a template for sequencing will go straight into our sequencing pipeline as soon as they are ready
- Unique bases can be used (see the website for Glen Research)

Synthesis scales and Cost:
Scale | Price per Base | Approximate Purified Yield |
---|---|---|
50 nmol | $0.60 | NOT AVAILABLE |
0.2 umol | $1.10 | 200-500 ug |
1.0 umol | $5.00 | 1-2 mg |
10 umol | $24.00 | 10 mg |
Degenerate Base List: (3' degeneracy would need a special column)
UPAC nucleotide code | Base |
---|---|
A | Adenine |
C | Cytosine |
G | Guanine |
T | Thymine |
R | A or G |
Y | C or T |
S | G or C |
W | A or T |
K | G or T |
M | A or C |
B | C or G or T |
D | A or G or T |
H | A or C or T |
V | A or C or G |
N | any base |
Please feel free to contact us: Lab: (410) 955-2739 | e-mail: syn_seq@jhmi.edu