DNA Synthesis and Purification
Oligonucleotide Synthesis Highlights:
- Oligos are synthesized and purified in our lab
- Turnaround time is usually the next day for crude oligos – depending on when the sequence is received
- HPLC purification is available
- We can help you design your primers at no charge but the oligos must be made in our lab.
- Oligo orders delivered with a template for sequencing will go straight into our sequencing pipeline as soon as they are ready
- Unique bases can be used (see the website for Glen Research)
Synthesis scales and Cost:
| Scale | Price per Base | Approximate Purified Yield |
|---|---|---|
| 50 nmol | $0.50 | NOT AVAILABLE |
| 0.2 umol | $1.00 | 200-500 ug |
| 1.0 umol | $5.00 | 1-2 mg |
| 10 umol | $24.00 | 10 mg |
Degenerate Base List: (3' degeneracy would need a special column)
| UPAC nucleotide code | Base |
|---|---|
| A | Adenine |
| C | Cytosine |
| G | Guanine |
| T | Thymine |
| R | A or G |
| Y | C or T |
| S | G or C |
| W | A or T |
| K | G or T |
| M | A or C |
| B | C or G or T |
| D | A or G or T |
| H | A or C or T |
| V | A or C or G |
| N | any base |
Please feel free to contact us: Lab: (410) 955-2739 | e-mail: syn_seq@jhmi.edu