Universal Primers

Universal Sequencing Primers



The Synthesis and Sequencing Facility will provide the following universal sequencing primers at no charge.

Primer Sequence


T7 Promotor

5' - TAA TAC GAC TCA CTA TAG G - 3'

T7 Terminator

5' - GCT AGT TAT TGC TCA GCG G - 3'

T3

5' - ATT AAC CCT CAC TAA AGG GA - 3'

M13 Rev

5' - CAG GAA ACA GCT ATG ACC - 3'

M13 For (-21) 

5' - TGT AAA ACG ACG GCC AGT - 3'

SP6

5' - GAT TTA GGT GAC ACT ATA G - 3'

PGex 3

5’ – CCG GGA GCT GCA TGT GTC AGA GG – 3’

PGex 5

5’ – GGG CTG GCA AGC CAC GTT TGG TG – 3’

BGH Rev

5’ – TAG AAG GCA CAG TCG AGG – 3’

pFastBacF

5’ – GGA TTA TTC ATA CCG TCC CA– 3’

pFastBacR

5’ – CAA ATG TGG TAT GGC TGA TT– 3’

PBAD For

5' - ATG CCA TAG CAT TTT TAT CC - 3'

pBAD Rev

5' - GAT TTA ATC TGT ATC AGG- 3'

Poly-T-V

5' - TTT TTT TTT TTT TTT TTT TTV- 3'

Poly-A-B

5' - AAA AAA AAA AAA AAA AAA B- 3'

CMV-For

5' - CGC AAA TGG GCG GTA GGC GTG- 3'

Please feel free to contact us: Lab: (410) 955-2739 | Fax: (410) 614-7566 | e-mail: syn_seq@jhmi.edu

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