Project Requests (sequencing long inserts)

DNA Sequencing Project Requests



Project Requests


Our Facility offers a free service of oligonucleotide design and contig construction. For each project we ask that you provide us with the following information:

Vector
Insert Length
Cloning Site
Double or Single Stranded Sequencing


We will then design primers using a program called "Oligo" for either single or double stranded projects and provide you with a consensus sequence and contig map when we are through. Your primers (40 nmole) will be returned to you labeled with both the primer and template names. Primers used in sequencing do not have to be purified, therefore, you will be receiving them in the crude form.

If you would like to request a project, please talk to the sequencing staff.

**Because this is a free service, we ask that you allow us to synthesize any oligos that we design.

Please feel free to contact us: Lab: (410) 955-2739 | Fax: (410) 614-7566 | e-mail: syn_seq@jhmi.edu

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