
Peptide Synthesis and Purification
Instrumentation consists of two Perkin Elmer/Applied Biosystems 433
large scale peptide synthesizers. The synthesizers are run using Fmoc
chemistry. Purification is performed by Reverse-phase HPLC.
Large Scale Synthesis Yields:
Expected yields are 200-400 mg of crude peptide and approximately
20-50 mg of purified peptide per each HPLC run consisting of a 150 mg
injection.
Cost:
Large Scale Synthesis:
$30.00 for each amino acid
$100.00 for each preparative HPLC
We will routinely accept peptides of 30 amino acids or less. Anything
greater than 30 will be accepted only after consultation.
Peptide Modifications:
There are many modifications available including, phosphorylated amino acids, N-terminal acetylation, N-terminal biotinylation, and N-terminal flourescent dyes. Please inquire about pricing. For any other unusual modifications, please see Jodie.
Peptides for Antibody Production:
The range of purity for peptides used for antibody production is
70% or greater. If your peptide is less than 70% pure, you can expect
approximately 20-50 mg after purification.
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