Peptide Synthesis and Purification

Instrumentation consists of two Perkin Elmer/Applied Biosystems 433 large scale peptide synthesizers. The synthesizers are run using Fmoc chemistry. Purification is performed by Reverse-phase HPLC.

Large Scale Synthesis Yields:

Expected yields are 200-400 mg of crude peptide and approximately 20-50 mg of purified peptide per each HPLC run consisting of a 150 mg injection.

Cost:

Large Scale Synthesis:

$30.00 for each amino acid
$100.00 for each preparative HPLC

We will routinely accept peptides of 30 amino acids or less. Anything greater than 30 will be accepted only after consultation.

Peptide Modifications:

There are many modifications available including, phosphorylated amino acids, N-terminal acetylation, N-terminal biotinylation, and N-terminal flourescent dyes. Please inquire about pricing. For any other unusual modifications, please see Jodie.

Peptides for Antibody Production:

The range of purity for peptides used for antibody production is 70% or greater. If your peptide is less than 70% pure, you can expect approximately 20-50 mg after purification.

 

Please feel free to contact us: Lab: (410) 955-2739 | Fax: (410) 614-7566 | e-mail: syn_seq@jhmi.edu

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