Overview:

Instrumentation consists of an Applied Biosystems 3730xl DNA Analyzer.

NEWS: 

• We are offering Ampure PCR clean-up using our robot for 50 cents a sample.

If you drop your sample off by 1:00 p.m., you can expect your data to be ready the next day. Please look at the white board when you drop off your samples for the date your data will be ready.  The date listed is the worse case scenario, it is always a good idea to check the server the next day in the afternoon anyway no matter what time you drop your samples off. 

We are also now offering Saturday delivery of your data to the server.

Sequencing Cost:  $6.00 per reaction (includes universal primer -- see universal primer link below for a complete list of our free primers.  We can add to the list if there is a primer that you think we should stock.)

Note:  We do not charge extra for any of our services except if your sample is a BAC/COSMID. 

Campus Sample Drop-Off Locations:

We have three drop-off locations located on campus. 

WBSB:  Outside of Room 418 for after hours.

BRB:  Outside of room 372

SOPH:  Inside the Monument street entrance where the FedEx box is located.  Look for the black "mailbox" on the wall.  Instructions are on the box.

Requested Concentrations:

Custom Primers Supplied by You:   4.0 uM in water with a total of 7 ul per reaction

Plasmid Templates:   0.1 to 0.2 ug/ul for (ds) plasmids with 7 ul per reaction in water.

ShRNAi Templates:  0.2 ug/ul with 10 ul per reaction in water.  DO NOT MIX WITH PRIMER, please provide template and primer separately if using custom primers.

PCR Products:  10 to 15 ng/ul (with an average length of 500 bp) adjust amount accordingly for longer PCR products.  If your PCR products are large, i.e., 4 Kb or something like that, then use the amounts requested under Plasmid Template.

BACs and COSMIDs:  0.5 ug/ul  with 12 ul per reaction in water.  Must be very clean.  The charge for this is $10.00 per reaction.  DO NOT MIX WITH PRIMER.

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The benefits of using YOUR JHMI Sequencing Facility:

• First and foremost, we are here for YOU.  This is the sole reason the facility exists.  So please make use of your Hopkins facility. 
• Template and Primer can be mixed by you or we can do it here in the lab. There is no extra charge if we mix it.  If mixing, use equal amounts. 
• We also offer other discounted pricing for samples that are already cycle sequenced by you.  Ask the staff for more details. 
• All samples will be stored for a month.  We will store your samples longer if you request this.  We also will hold on to primers that you use frequently as well. 
• Every chromatogram is analyzed by hand after printing.  We will repeat samples, at our discretion, should we see that something failed when everything else worked. 
• We are available to talk with you from 7:30 a.m.  to 5:00 p.m. Monday through Friday regarding your sequencing.  Email is checked over the weekend as well; and if possible, we will try to resolve any problems you may have.
  
  
  Free DNA Sequencing Chromatogram Viewers:

   Geospiza FinchTV

FinchTV can be used for both PCs and Macs.  When they send the link, it may end up in your Junk Mail folder so be sure to look there. 

   Applied Biosystems

       Sequence Scanner

For more detailed information please select one of the following:

Plasmid Template Preparation

Retrieving Data From the Server

Universal Primers

Project Requests (sequencing long inserts)