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M. Daniel Lane
Distinguished Service Professor of Biological Chemistry
Johns Hopkins University School of Medicine
JHU School of Medicine
725 N. Wolfe St. 512 WBSB
BALTIMORE, MD 21205
office tel: 410-955-3554
lab tel: 410-955-3975
Fax: 410-955-0903
Email: dlane@jhmi.edu
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| Signals/genes that trigger stem cell/adipose lineage commitment and differentiation; Hypothalamic control of adiposity/obesity |
| Our research goals are to determine the mechanisms:
1. by which mesenchymal stem cells commit to the adipocyte lineage and differentiate into adipocytes. We utilize mouse pluripotent stem and preadipocyte cell lines as models that faithfully mimic commitment and differentiation in vivo. We focus on the signaling and gene transcription cascades that trigger these processes, and
2. by which whole body energy status is monitored by the hypothalamus and how this information is transmitted to higher brain centers to suppress food intake. We discovered that the hypothalamic malonyl-CoA, a biochemical indicator of energy surplus, reciprocally alters orexigenic and anorexigenic neuropeptide expression and thereby, appetite.
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Wolfgang, M.J., Kurama,T., Dai, Y., Suwa, Y., Asaumi, M., Matsumoto, S., Cha, S.H., Shimokawa, T. and Lane, M.D. (2006) The brain-specific carnitine palmitoyltransferase-1c regulates energy homeostasis, Proc. Natl. Acad. Sci. USA. 103, 7282¡V7287.
PubMed Reference
Bowers, R.R., Kim, J.W., Otto, T.C. and Lane, M.D. (2006) Stable stem cell commitment to the adipocyte lineage by inhibition of DNA methylation: Role of the BMP-4 gene. Proc. Natl. Acad. Sci. USA. 103, 13,022-13,027.
PubMed Reference
Cha, S.H., Rodgers, J.T., Puigserver, P., Chohnan, S. and Lane, M.D. (2006) Hypothalamic malonyl-CoA triggers mitochondrial biogenesis and oxidative gene expression in skeletal muscle: Role of PGC1Ą. Proc. Natl. Acad. Sci. USA. 103, 15,410-15,415.
PubMed Reference
Bowers, R.R., Kim, J.W., Otto, T.C. and Lane, M.D. (2006) Stable stem cell commitment to the adipocyte lineage by inhibition of DNA methylation: Role of the BMP-4 gene. Proc. Natl. Acad. Sci. USA. 103, 13,022-13,027.
Kim. J.-W., Tang, Q.-Q., Li, X. and Lane, M.D. (2007) Effect of phosphorylation and S-S bond-induced dimerization on DNA binding and transcriptional activation by C/EBPƒÒ. Proc. Natl. Acad. Sci. USA, 1800-1804.
PubMed Reference
Bowers, R.R. and Lane, M.D. (2007) A role for bone morphogenetic protein-4 in adipocyte development. Cell Cycle 6, 385-389
PubMed Reference
Chakravarthy, M.V., Zhu, Y., Lopez, M.M., Yin, L., Wozniak. D.F, Coleman, T., Hu, Z., Wolfgang, M.J., Antonio Vidal-Puig, A., Lane, M.D. and Semenkovich, C.F. (2007) Brain fatty acid synthase activates PPARa to maintain energy homeostasis.J. Clin. Invest., 117, 2539-2552.
PubMed Reference
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