The Englund Laboratory
Department of Biological Chemistry - Johns Hopkins University School of Medicine

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Englund Lab Publications - 1990 to Present
 

For a complete list of publications see Englund CV

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Autio, K.J., Guler, J.L., Kastaniotis, A.J., Englund, P.T. and Hiltunen, J.K. (2008) The 3-hydroxyacyl-ACP dehydratase of mitochondrial fatty acid synthesis in Trypanosoma brucei.
FEBS Lett. 2008 Mar 5;582(5):729-33. Epub 2008 Feb 5.

Guler, J.L., Kriegova, E., Smith, T.K., Lukeš, J. and Englund, P.T. (2008) Mitochondrial fatty acid synthesis is required for normal mitochondrial morphology and function in Trypanosoma brucei.
Mol Microbiol. 67(5):1125-42. Epub 2008 Jan 23

Roy Chowdhury, A., Zhao, Z. and Englund, P.T. (2008) Effect of hydroxyurea on procyclic Trypanosoma brucei: An unconventional mechanism for achieving synchronous growth. Eukaryot Cell. 7(2): 425-8. Epub 2007 Dec 14.

Zhao, Z., Lindsay, M.E., Roy Chowdhury, A., Robinson, D.R. and Englund, P.T. (2008) p166, a link between the trypanosome mitochondrial DNA and flagellum, mediates genome segregation. EMBO J. 27(1): 143-54. Epub 2007 Dec 6.

Milman, N., Motyka, S.A., Englund, P.T., Robinson, D. and Shlomai, J. (2007) Mitochondrial origin-binding protein UMSBP mediates DNA replication and segregation in trypanosomes. Proc Natl Acad Sci U S A. 104(49):19250-5. Epub 2007 Nov 28.

Liu, Y. and Englund, P.T. (2007) The rotational dynamics of kinetoplast DNA replication.
Mol Microbiol. 64(3):676-90.

Lee, S.H., Stephens, J.L. and Englund, P.T.(2007) A fatty-acid synthesis mechanism specialized for parasitism. Nat Rev Microbiol. 5(4):287-97.

Stephens, J.L., Lee, S.H., Paul, K.S. and Englund, P.T. (2007) Mitochondrial fatty acid synthesis in Trypanosoma brucei. J Biol Chem. 282(7):4427-36. Epub 2006 Dec 12.

Lee, S.H., Stephens, J.L., Paul, K.S. and Englund, P.T. (2006) Fatty acid synthesis by elongases in trypanosomes. Cell. 126(4):691-9.

Liu, B., Molina, H., Kalume, D., Pandey, A., Griffith, J.D. and Englund, P.T. (2006) Role of p38 in replication of Trypanosoma brucei kinetoplast DNA. Mol Cell Biol. 26(14):5382-93.

Motyka, S.A., Drew, M.E., Yildirir, G. and Englund, P.T. (2006) Overexpression of a cytochrome b5 reductase-like protein causes kinetoplast DNA loss in Trypanosoma brucei. J Biol Chem. 281(27):18499-506. Epub 2006 May 10.

Englund, P.T., Agbo, E.E., Lindsay, M.E., Liu, B., Liu, Y., Motyka, S.A., Yildirir, G. and Zhao, Z. (2005) RNAi libraries and kinetoplast DNA. Biochem Soc Trans. 33(Pt 6):1409-12.

Liu, Y., Motyka, S.A. and Englund, P.T.(2005) Effects of RNA interference of Trypanosoma brucei structure-specific endonuclease-I on kinetoplast DNA replication. J Biol Chem. 280(42):35513-20. Epub 2005 Aug 11.

Liu, B., Liu, Y., Motyka, S.A., Agbo, E.E. and Englund, P.T. (2005) Fellowship of the rings: the replication of kinetoplast DNA. Trends Parasitol. 21(8):363-9.

Paul, K.S., Bacchi, C.J. and Englund, P.T. (2004) Multiple triclosan targets in Trypanosoma brucei. Eukaryotic Cell, 3(4): 855-61.

Acosta-Serrano, A., O'Rear, J., Quellhorst, G., Lee, S.H., Hwa, K-Y, Krag, S.S., and Englund, P.T. (2004) Defects in the N-Linked Oligosaccharide Biosynthetic Pathway in a Trypanosoma brucei Glycosylation Mutant. Eukaryotic Cell, 3(2), 255-63.

Motyka, S.A. and Englund, P.T. (2004) RNA interference for analysis of gene function in trypanosomatids. Current Opinion in Microbiology, 7(4):362-8.

Jiang, , D.W., Werbovetz, K.A., Varadhachary, A., Cole, R.,N., and Englund, P.T. (2004) Purification and identification of a fatty acyl-CoA synthetase from Trypanosoma brucei. Mol. Biochem. Parasitol. 135(1), 149-52.

Klingbeil M.M., Englund P.T. (2004) Closing the gaps in kinetoplast DNA network replication.
Proc Natl Acad Sci U S A. 101(13):4333-4.

Motyka, S.A., Zhao, Z, Gull, K., and Englund, P.T. (2004) Alternative integration of pZJM library plasmids in Trypanosoma brucei. Mol. Biochem. Parasitol. 2004 Mar;134(1):163-7.

Drew, M.E., Motyka, S.A., Morris, J.C., Wang, Z., and Englund, P.T. (2004) Inducible RNAi as a forward genetic tool in Trypanosoma brucei. In: RNA Interference: From Basic Science to Drug Development (Appasani, K. Ed.) Cambridge University Press, Cambridge, U.K. 247-56.

Morris, J., Wang,, Z, Motyka, S.A., Drew, M.E., Englund, P.T. (2004) An RNAi-based genomic library for forward genetics in the African trypanosome. In: Gene Silencing by RNA Interference: Technology and Application. (Sohail, M., Ed.) Boca Raton: CRC Press LLC, 241-57.

Saxowsky, T.T., Choudhary, G, Klingbeil, M.M., and Englund, P.T. (2003) Trypanosoma brucei has two distinct mitochondrial DNA polymerase beta enzymes. J. Biol. Chem. 278, 49095-49101.

Drew, M.E., Morris, J.C., Wang, Z., Wells, L., Sanchez, M, Landfear, S.M., and Englund, P.T. (2003) The adenosine analog tubercidin inhibits glycolysis in Trypanosoma brucei as revealed by an RNAi library. J. Biol. Chem. 278, 46596-46600.

Liniger, M., Acosta-Serrano, A., Van Den Abbeele, J., Kunz Renggli, C., Brun, R., Englund, P.T., Roditi, I. (2003) Cleavage of trypanosome surface glycoproteins by alkaline trypsin-like enzyme(s) in the midgut of Glossina morsitans Int. J. Parasitol. 33, 1319-1328.

Saxowsky, T.T., Matsumoto, Y., and Englund, P.T. (2002) The mitochondrial DNA polymerase beta from Crithidia fasciculata has 5'-deoxyribose phosphaae (dRP) lyase activity but is deficient in the release of dRP. J. Biol. Chem. 277, 37201-37206.

Wang, Z., Drew, M.E., Morris, J.C., and Englund, P.T. (2002) Asymmetrical division of the trypanosome's kinetoplast DNA network. EMBO J. 21,4998-5005.

Morris, J.C..,Wang, Z., Drew, M.E., and Englund, P.T. (2002) Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi library. EMBO J. 21, 4429-4438.

Lukes, J., Guilbride, D.L., Votypka, J., Zikova, A., Benne, R., and Englund, P.T. (2002) Kinetoplast DNA network: Evolution of an improbable structure. Eukaryotic Cell, 1, 495-502.

Klingbeil, M.M., Motyka, S.A., and Englund, P.T. (2002) Multiple mitochondrial DNA polymerases in Trypanosoma brucei. Molecular Cell. 10, 175-186.

Grams, J., Morris, J.C., Drew, M.E., Wang, Z., Englund, P.T., and Hajduk, S.L. (2002) A trypanosome mitochondrial RNA polymerase is required for transcription and replication. J. Biol. Chem. 277,16952 -16959.

Klingbeil, M.M., Drew, M.E., Liu, Y., Morris, J.C., Motyka, S.A., Saxowsky, T.T., Wang, Z., and Englund, P.T. (2001) Unlocking the secrets of trypanosome kinetoplast DNA network replication. The Protist 15. 2,255 - 262.

Wang, Z. and Englund, P.T. (2001) RNA interference of a trypanosome topoisomerase II causes progressive loss of mitochondrial DNA. EMBO J. 20, 4674-4683.

Doering, T.L., Englund, P.T. and Hart, G.W. (2001) Detection of glycophospholipid anchors on proteins.
Curr Protoc Mol Biol. 2001 May;Chapter 17:Unit17.8.

Morris, J.C., Wang, Z., Drew, M.E., Paul, K.S., and Englund, P.T. (2001) Inhibition of bloodstream form Trypanosoma brucei gene expression by RNA interference using the pZJM dual T7 vector. Mol. Biochem. Parasitol, 117, 111-113.

Vassella, E., Acosta-Serrano, A., Studer, E., Lee, S.H., Englund, P.T., and Roditi, I. (2001) Multiple procyclin isoforms are differentially expressed during development of insect forms of Trypanosoma brucei. J. Mol. Biol. 312, 597-307.

Paul, K.S., Jiang, D., Morita, Y.S., and Englund, P.T. (2001) Fatty acid synthesis in African trypanosomes: A solution to the myristate mystery. Trends in Parasitology. 17, 381-387.

Abu-Elneel, K, Robinson, D.R., Drew, M.E., Englund, P.T., and Shlomai, J. (2001) Intramitochondrial localization of universal minicircle sequence-binding protein, a trypanosomatid protein that binds kinetoplast minicircle replication origins. J. Cell Biol. 153, 725-733.

Drew, M.E. and Englund, P.T. (2001) Intramitochondrial location and dynamics of Crithidia fasciculata kinetoplast minicircle replication intermediates. J. Cell Biol. 153, 735-743.

Acosta-Serrano, A, Vassella, E., Liniger, M. Renggli, C.K., Brun, R., Roditi, I., and Englund, P.T. (2001) The surface coat of procyclic Trypanosoma brucei: programmed expression and proteolytic cleavage of procyclin in the tsetse fly. Proc. Natl. Acad. Sci. USA, 98, 1513-1518.

Morita, Y.S. and Englund, P.T. (2001) Fatty acid remodeling of glycosyl phosphatidylinositol anchors in Trypanosoma brucei: incorporation of fatty acids other than myristate. Mol. Biochem. Parasitol. 115, 157-164.

Leal, S., Acosta-Serrano, A., Morita, Y.S., Englund, P.T., Böhme, U, and Cross, G.A.M. (2001) Virulence of Trypanosoma brucei strain 427 is not affected by the absence of glycosylphosphatidylinositol phospholipase C. Mol. Biochem. Parasitol. 114, 245-247 (2001).

Morris, J.C., Drew, M.E., Klingbeil, M.M., Motyka, S.A., Saxowsky, T.T., Wang, Z, and Englund, P.T. (2000). Replication of kinetoplast DNA: An update for the new millenium. Int. J. Parasitol. 31,453-458

Wang, Z, Morris, J.C., Drew, M.E., and Englund, P.T. (2000) Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J. Biol. Chem. 275, 40174-40179.

Acosta-Serrano, A., Cole, R.N., and Englund, P.T. (2000) Killing of Trypanosoma brucei by Concanavalin A: Structural Basis of Resistance in Glycosylation Mutants. J. Mol. Biol. 304, 633-644.

Pearson, T.W., Beecroft, R.P., Welburn, S.C., Ruepp, S., Roditi, I, Hwa, K-Y., Englund, P.T., Wells, C.W., and Murphy, N.B. (2000) The major cell surface glycoprotein procyclin is a receptor for induction of a novel form of cell death in African trypanosomes in vitro. Mol. Biochem. Parasitol. 111, 333-349.

Morita, Y.S., Acosta-Serrano, A., Buxbaum, L.U., and Englund, P.T. (2000) Glycosyl phosphatidylinositol myristoylation in African trypanosomes: new intermediates in the pathway for fatty acid remodeling. J. Biol. Chem. 275, 14147-14154.

Jiang, D.W., Ingersoll, R., Myler, P.J., and Englund, P.T. (2000) Four tandemly-linked long chain fatty acyl-CoA synthetases in Trypanosoma brucei. Experimental Parasitology 96,16-22.

Morita, Y.S., Paul, K.S., and Englund, P.T. (2000) Specialized fatty acid synthesis in African trypanosomes: Myristate for GPI anchors. Science. 288, 140-143.

Morita, Y.S., Acosta-Serrano, A., and Englund, P.T. (2000) The biosynthesis of GPI anchors. in Oligosaccharides in Chemistry and Biology, (eds. Ernst, B., Sinay, B, and Hart, G.), Wiley-VCH. Weinheim, Germany. P. 417-433.

Acosta-Serrano, A., Cole, R.N., Mehlert, A., Lee, M.G.-S., Ferguson, M.A.J., and Englund, P.T. (1999) The procyclin repertoire of Trypanosoma brucei: Identification and structural characterization of the EP-rich polypeptides. J. Biol. Chem. 274, 29763-29771.

Johnson, C.E. and Englund, P.T. (1999) A refined localization of the mitochondrial DNA primase in Crithidia fasciculata. Mol. Biochem. Parasitol. 102, 205-208.

Werbovetz, K.A. and Englund, P.T. (1999) Glycosyl phosphatidylinositol myristoylation in African trypanosomes. in The trypanosome surface. Proceedings of the Third Francqui Colloquum, 26-27 May, 1997, Brussels (E. Pays, ed.) Bibliotheque Scientifique Francqui (De Boeck Universite). 207-216.

Shapiro, T.A., Klein, V.A., and Englund, P.T. (1999) Isolation of kinetoplast DNA, in Protocols for DNA topology and topoisomerases, Part 1: DNA topology and enzymes. (M.A. Bjornsti and N. Osheroff, eds.) Humana Press Inc. Totowa, NJ 61-67.

Hwa, K-Y., Acosta-Serrano, A., Khoo, K-H., Pearson, T.W., and Englund, P.T. (1999) Protein glycosylation mutants of procyclic Trypanosoma brucei: Defects in the asparagine-glycosylation pathway. Glycobiology, 9, 181-190.

Milne, K.G., Ferguson, M.A.J., and Englund, P.T. (1999) A novel glycosylphosphatidylinositol in African trypanosomes: a possible catabolic intermediate. J. Biol. Chem. 274, 1465-1471.

Johnson, C.E., and Englund, P.T. (1998) Changes in organization of Crithidia fasciculata kinetoplast DNA replication proteins during the cell cycle. J. Cell Biol. 143, 911-919.

Guilbride, D.L. and Englund, P.T. (1998) Kinetoplast DNA: the amazing mitochondrial DNA in trypanosomes. South African Journal of Science, 94, 273-275.

Guilbride, D.L. and Englund, P.T. (1998) The replication mechanism of kinetoplast DNA networks in several trypanosomatid species. J. Cell Science 111, 675-679.

Gazzinelli, R.T., Camargo, M.M., Almeida, I.C., Morita, Y.S., Giraldo, M., Acosta-Serrano, A., Hieny, S., Englund, P.T., Ferguson, M.A.J., Travassos, L.R., and Sher, A. (1997) Identification and characterization of protozoan products that trigger the synthesis of IL-12 by inflammatory macrophages. Chemical Immunology, 68, 136-152.

Li, C. and Englund, P.T. (1997) A mitochondrial DNA primase from the trypanosomatid Crithidia fasciculata. J. Biol. Chem. 272, 20787-20792.

Werbovetz, K.A. and Englund, P.T. (1997) Glycosyl phosphatidylinositol myristoylation in African trypanosomes. Mol. Biochem. Parasitol. 85, 1-7.

Werbovetz, K.A., Bacchi, C.J., and Englund, P.T. (1996) Trypanocidal analogs of myristate and myristoyllysophosphatidylcholine. Mol. Biochem. Parasitol. 81, 115-118.

Werbovetz, K.A. and Englund, P.T. (1996) Lipid metabolism in Trypanosoma brucei: Utilization of myristate and myristoyllyso phosphatidylcholine for myristoylation of glycosyl phosphatidylinositols. Biochem. J. 318, 575-581.

Carpenter, L.R. and Englund, P.T. (1996) Linearized free maxicircle DNA in Crithidia fasciculata is a product of topoisomerase II-mediated cleavage. Mol. Biochem. Parasitol. 76, 115-123.

Torri, A.F., Rocco, L.J., and Englund, P.T. (1996) The replication of kinetoplast DNA. in DNA replication in eukaryotic cells. (M. De Pamphilis, ed.) Cold Spring Harbor Laboratory Press. Cold Spring Harbor pp 1029-1042.

Englund, P.T., Guilbride, D.L., Hwa, K-Y., Johnson, C.E., Li, C., Rocco, L.J., and Torri, A.F. (1996) Kinetoplast DNA: structure and replication. in Molecular Biology of Parasitic Protozoa (D. Smith and M. Parsons, eds.) Oxford University Press pp. 75-87.

Shapiro, T.A. and Englund, P.T. (1995) The structure and replication of kinetoplast DNA. Annu. Rev. Microbiol. 49, 117-143.

Torri, A.F. and Englund, P.T. (1995) A DNA polymerase beta in the mitochondrion of the trypanosomatid Crithidia fasciculata. J. Biol. Chem. 270, 3495-3497.

Buxbaum, L.U., Raper, J., Opperdoes, F.R., and Englund, P.T. (1994) Myristate exchange: A second glycosyl phosphatidylinositol myristoylation reaction in African trypanosomes. J. Biol. Chem. 269, 30212-30220.

Chen, J., Rauch, C.A., White, J.H., Englund, P.T., and Cozzarelli, N.R., (1995) The topology of the kinetoplast DNA network. Cell 80, 61 - 69.

Ferguson, M.L., Torri, A.F., Pérez-Morga, D.L., Ward, D.C., and Englund, P.T. (1994) Kinetoplast DNA replication: Mechanistic differences between Trypanosoma brucei and Crithidia fasciculata. J. Cell Biol. 126, 631-639.

Doering, T.L., Lu, T., Werbovetz, K.A. Gokel, G.W., Hart, G.W., Gordon, J.I., and Englund, P.T. (1994) The toxicity of myristic acid analogs towards African trypanosomes. Proc. Nat Acad. Sci. USA. 91, 9735-9739.

Doering, T.L., Pessin, M.S., Hart, G.W., Raben, D.M. and Englund, P.T. (1994) The fatty acids in unremodeled trypanosome glycosyl phosphatidylinositols. Biochem. J., 299, 741-746.

Mensa-Wilmot, K., Morris, J.C., Al-Qahtani, A., and Englund, P.T. (1995) Purification and use of recombinant glycosylphosphatidylinositol phospholipase C. Meth. Enzymol., 250, 641-655.

Englund, P.T., Ferguson, M., Guilbride, D.L., Johnson, C.E., Li, C., Pérez-Morga, D., Rocco, L.J., and Torri, A.F. (1995) The replication of kinetoplast DNA. In: Molecular Approaches to Parasitology (J.C. Boothroyd and R. Komuniecki, eds.), Wiley-Liss, New York, pp 147-161.

Pérez-Morga, D. and Englund P.T. (1993) Kinetoplast DNA. Current Biology 3, 529.

Torri, A.F., Kunkel, T.A., and Englund, P.T. (1994) A beta-like DNA polymerase from the mitochondrion of the trypanosomatid Crithidia fasciculata. J. Biol. Chem., 269,8165-8171.

Pérez-Morga, D. and Englund P.T. (1993) The structure of replicating kinetoplast DNA networks. J. Cell Biol. 123, 1069-1079.

Englund, P.T. (1993) Myristoylation of the trypanosome variant surface glycoprotein: A possible target for chemotherapy. In: Biotechnology: Theory and Applications (W. Tien et al, eds), Development Center for Biotechnology, Taipei, Taiwan, pp. 36-42.

Pérez-Morga, D.L. and Englund, P.T. (1993) The attachment of minicircles to kinetoplast DNA networks during replication. Cell 74, 703-711.

Raper, J., Doering, T.L., Buxbaum, L.U. and Englund P.T. (1993) A review. Glycosyl phosphatidylinositols in Trypanosoma brucei. Experimental Parasitology 76, 216-220.

Doering, T.L., Pessin, M.S., Hoff, E.F., Hart, G.W., Raben, D.M. and Englund, P.T. (1993) Trypanosome metabolism of myristate, the fatty acid required for the variant surface glycoprotein membrane anchor. J. Biol. Chem. 268, 9215-9222.

Pérez-Morga, D.L. and Englund, P.T. (1993) Microtechnique for electron microscopy of DNA. Nucleic Acids Research 21, 1327-1328.

Rauch, C.A., Pérez-Morga, D.L., Cozzarelli, N.R., and Englund, P.T. (1993) The absence of supercoiling in kinetoplast DNA minicircles. EMBO J. 12, 403-411.

Effron, P.N., Torri, A.F., Engman D.M., Donelson, J.E. and Englund, P.T. (1993) A mitochondrial heat shock protein from Crithidia fasciculata. Mol. Biochem. Parasitol. 59, 191-200.

Doering, T.L., Englund, P.T. and Hart, G.W. (1993). Detection of glycophospholipid protein anchors. In: Current Protocols in Molecular Biology, F.M. Ausubel et al, Eds., Unit 17, Chapter 17, Analysis of Glycoconjugates, Guest Eds. Varki, A. and Coligan J., Greene Publishing & Wiley-Interscience, NY, Supplement 22, pp. 17.8.1-17.8.13.

Englund, P.T. (1993) The structure and biosynthesis of glycosyl phosphatidylinositol protein anchors. Ann. Rev. Biochem. 62, 121-138.

Mensa-Wilmot, K. and Englund, P.T. (1992) Glycosyl phosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Expression in E. coli. Mol. Biochem. Parasitol. 56, 311-322.

Ferguson, M., Torri, A.F., Ward, D.C. and Englund, P.T. (1992) In situ hybridization to the Crithidia fasciculata kinetoplast reveals two antipodal structures involved in kinetoplast DNA replication. Cell 70, 621-629.

Torri, A.F. and Englund, P.T. (1992) Purification of a mitochondrial DNA polymerase from Crithidia fasciculata. J. Biol. Chem. 267, 4786-4792.

Mensa-Wilmot, K. and Englund, P.T. (1992) Non-fusion protein expression in E. coli: detection by a-complementation (blue/white selection). Nucleic Acids Research 20, 143.

Mensa-Wilmot, K., Doering, T.L., Raper, J., Buxbaum, L., Hart, G.W. and Englund, P.T. (1991) Glycosyl phosphatidylinositol membrane anchors in African trypanosomes. In: Molecular & Immunological Aspects of Parasitism (C.C. Wang, ed), AAAS Press, Washington, D.C., 43-54.

Doering, T.L., Raper, J., Buxbaum, L.U., Adams, S.P., Gordon, J.I., Hart, G.W. and Englund, P.T. (1991) An analog of myristic acid with selective toxicity for African trypanosomes. Science 252, 1851-1854.

Doering, T.L., Raper, J., Buxbaum, L.U., Hart, G.W. and Englund, P.T. (1990) Biosynthesis of glycosyl phosphatidylinositol protein anchors. In: METHODS: A Companion to Methods in Enzymology 1, 288-296.

Shapiro, T.A. and Engund, P.T. (1990) Inhibition of type II topoisomerase activity in the mitochondria of trypanosomes. In: Chemotherapy for Trypanosomiasis (A. Peregrine, ed.), The International Laboratory for Research on Animal Diseases, Nairobi, Kenya, pp. 79-84.

Masterson, W.J., Raper, J., Doering, T.L., Hart, G.W. and Englund, P.T. (1990) Fatty acid remodeling: a novel reaction sequence in the biosynthesis of trypanosome glycosyl phosphatidylinositol membrane anchors. Cell 62: 73-80.

Doering, T.L., Masterson, W.J., Hart, G.W. and Englund, P.T. (1990) Biosynthesis of glycosyl-phosphatidylinositol membrane anchors. (Minireview) J. Biol. Chem. 265, 611-614.

Mensa-Wilmot, K., Hereld, D. and Englund, P.T. (1990) Genomic organization, chromosomal localization and developmentally regulated expression of the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Mol. Cell Biol. 10, 720-726

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